Hypoxanthine-xanthine oxidase down-regulates GLUT1 transcription via SIRT1 resulting in decreased glucose uptake in human placenta

M Lappas; S Andrikopoulos; M Permezel

Journal article

Hypoxanthine-xanthine oxidase down-regulates GLUT1 transcription via SIRT1 resulting in decreased glucose uptake in human placenta

M Lappas, S Andrikopoulos, M Permezel

Journal of Endocrinology | BIOSCIENTIFICA LTD | Published : 2012

DOI: 10.1530/JOE-11-0355

Abstract

Appropriate foetal growth and development is dependent on adequate placental glucose uptake. Oxidative stress regulates glucose uptake in various tissues. The effect of oxidative stress on placental glucose transport is not known. Thus, the aim of this study was to determine the effect of oxidative stress on glucose uptake and glucose transporters (GLUTs) in human placenta. Human placenta was incubated in the absence or presence of 0.5mM hypoxanthineC15mU/ml xanthine oxidase (HX/XO) for 24h. Gene and protein expressions of the GLUTs were analysed by quantitative RT-PCR and western blotting respectively. Glucose uptake was measured using radiolabelled (14C) glucose. HX/XO significantly decrea..

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University of Melbourne Researchers

Martha Lappas Author

Sof Andrikopoulos Author

Michael Permezel Author

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Grants

Awarded by National Health and Medical Research Council (NHMRC)

Funding Acknowledgements

Dr M L was a recipient of a National Health and Medical Research Council (NHMRC) RD Wright Fellowship (grant no. 454777). The work described in this manuscript was, in part, funded by a project grant from NHMRC (grant no. 454310). Funding for the iQ5 multicolour RT-PCR machine (in-part), Chemi-Doc system and xMark Microplate Absorbance Spectrophotometer was provided by the Medical Research Foundation for Women and Babies.